Trademark not copyright. Copyright only applies to entire works like a song text or the code Twitter has produced. For example the song title alone is not copyrighted. A trademark realistically only expires when it gets contested in court.
There's a similar law where you must at least defend a trademark against misuse to keep hold of it. That was the reasoning behind this music video for Velcro:
So, based on that, maybe they won't lose it just by not using it. But if someone else tried to establish a Twitter product, they may lose the trademark if they don't fight it.