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I have never met anyone in real life that doesn't address you the way you ask them to. My language, however, does not have gender neutral pronouns so the "did you just assume X gender" question is kinda annoying.
I definitely have, just last week my girlfriend got intentionally misgendered multiple times while going to get her gender marker changed, by a social security agent. Some people can be incredibly cruel like that. An overwhelming majority of people are not like that though, in my experience, but the sting of cruelty is louder than passive acceptance.
Like this is a question that people ask and not a troll ragebait. People most positively misgender on purpose upon inspection that a person is trans. If a cis person says "but I am a boy" they will bend over apologizing.